tielfan wrote:When vitamin B12 is eaten in food it is released in the ventriculus/gizzard and absorbed in the small intestine. But the production site is in the colon, which is further down the line. That's why Klasing says "A diet almost completely devoid of animal foods and managements practices that prevent coprophagy [poop eating] are necessary to demonstrate a deficiency in poultry and Japanese Quail." If you do a google search for B12 feces you'll find a number of sites saying that vegans can get vitamin B12 by eating their own feces. There are even some sites contending that there's enough production higher up that a few vegans may be able to get by without this type of supplementation, but that's not typical.
I'm not qualified to evaluate the liver results of the 70% protein diet and I doubt that you are either. So all I can tell you is that every professional-level citation I've seen of this study indicated that the results were not considered to be a problem. There wasn't anyone who said "The kidneys do fine on this diet but oh my god you should see how it messes up the liver." If you know of a respectable source that does say something along those lines I would appreciate seeing it, because I want to know these things. The concerns about the dangers of a high protein diet relate primarily to kidney damage, which appears to not actually be a problem for cockatiels at least. Liver disease from protein consumption is generally not considered to be risk in healthy individuals, although people/birds who already have liver disease have to watch their intake.
The safety or not of a 70% protein intake is a moot point anyway. Nobody expects a diet that abnormal to be healthy in the long run. The question is about normal consumption levels The idea expressed in this thread that the protein level in seeds is dangerously high is quite unique - in fact I don't think I've ever seen it before. The mainstream complaint about "birdseed" is that it's too high in either carbohydrates or fat, and too low in protein. I've already presented professional statements about protein deficiency caused by an all-seed diet and links showing that the amino acid scores for seeds and grains indicate a protein level that's lower than the ideal protein to calorie ratio. I've also never seen anyone say before that the protein in seeds is a cause of fatty liver disease. There's plenty of complaints about the amount of fat in sunflower seed (which is more justifiable), but when it comes to protein all the sources that I've seen indicate that a protein deficiency can be a cause of fatty liver disease. For example:
http://www.gastrojournal.org/article/S0 ... 7/abstractThe mainstream view isn't always right of course, but if you're going to swim against the mainstream you need to have some convincing evidence that you are right and they are wrong. You've been very dismissive of everything I've had to say, and at this point it would be very helpful for you to provide some evidence that seed really is excessively high in protein no matter what the professional avian nutrition community and the general-purpose nutritional databanks have to say about it.
It would also be helpful to have some calculations showing that the recipe in this thread is actually lower in complete protein than a standard birdseed mix, because I don't see how it can be (unless there is evidence that combining beans with grains actually lowers the protein level instead of increasing it). Some of the grains in this recipe are lower in total amino acids than millet and canarygrass seed (two of the standard ingredients in a basic birdseed mix), but they make up for it with higher protein quality scores. Oats are a common ingredient in both the birdseed mix and the recipe, and it has higher levels of protein than most grains along with a high protein quality score.
Nutrition Data's protein quality scores:
Basic birdseed mix:
Millet Protein per 100g: 4 grams Amino acid score 38
Canarygrass (NOT from NutritionData; accuracy of information is questionable
Protein per 100g: 22 grams Amino acid score 37
Cereal oats, not fortified dry Protein per 100g: 13 grams Amino acid score 95
Recipe ingredients:
Kamut Protein per 100g: 2 grams Amino acid score 55
Wheat, durum Protein per 100g: 2 grams Amino acid score 43
Barley, hulled Protein per 100g: 2 grams Amino acid score 73
Cereal oats, not fortified dry Protein per 100g: 13 grams Amino acid score 95
Rice, brown long-grain raw Protein per 100g: 3 grams Amino acid score 75
Corn, yellow Protein per 100g: grams Amino acid score 55
Hominy, canned white Protein per 100g: 1 grams Amino acid score 44
Flaxseed Protein per 100g: 18 grams Amino acid score 92
White beans, canned Protein per 100g: 7 grams Amino acid score 104
OK, let's see. B12 produced in the colon - can you give me a link for this info? Because everything I have and looked up before I answered says that although humans make it in the small intestine, herbivores either use the rumen or their stomach(s) (insects and birds do it this way - did you read the biologist reply to the question for which I provided the link?)
Klasing quote - he is talking about a species that is omnivore and another one that is herbivore/insectivore and we are mostly talking about parrot species that are classified as herbivore. There is a difference - for example, we, humans, are omnivores and because we are supposed to eat meat (which has B12) we don't produce enough of our own (insect meat is also very rich in B12) so animals that evolved to eat a diet that already has B12 in it, were not 'made' to produce enough of it.
Analyzing studies - yes, you are correct, it's not easy and simply reading the conclusions doesn't do it, either. You had concluded that cockatiels do 'just fine' on a 70% protein diet and, apparently, Brandon must have read a posting of yours stating so, repeated the info here and, when I knocked it down, told you that there was somebody here that did not agree with you so you came to see why -which I celebrate because I love people who go to scientific studies to learn instead of just reading what somebody posted in a bird site! (This is what I ALWAYS tell people to do so you are a parrot keeper after my own heart

). Now, I have no formal education on avian nutrition, all I know is self-taught and you are right that you need to be very careful about studies. I learned this myself over 20 years of reading studies with a dictionary next to me

Let me share with you what I've learned:
1. You need to look at the purpose of the study because the conclusion is going to be specific to it (in this one, they wanted to find out at what level protein became toxic -unfortunately, they did not make the distinction of acute toxicity versus chronic toxicity and that's where our disagreement stems from because there are substances that might not be acutely toxic but that, consumed in large quantities, become chronically toxic -like sugar, for example)
2. You need to look at the subjects (in this case it's perfect because it's parrots but, for example, you used estrogen and testosterone production in humans as an example that high protein does not influence them which is not good because humans reproductive system is completely different from the avian - we produce sexual hormones all the time, birds don't - we stop producing them as we get old, birds don't - mammals have monoestrus cycles, birds are photoperiodic, etc)
3. You need to look at the methodology (and this includes duration - in this case, it was only 11 months done on young, healthy birds that were killed after so as to perform necropsies)
4. You need to look at ALL the information (in this case it would be weight, uric acid, liver lesions, granulomas, etc)
Protein level in seeds - I might be wrong but I don't think anybody here ever said that the level of protein in seed, per se, is too high (I feed seed and would not do it if I though it was bad for my birds), what we might have said is that free-feeding high protein food is bad. And the reason for this is that parrots are 'programmed' to gorge on protein food the same way that humans are 'programmed' to gorge on, say, salt, for example. Nature made it so all animals crave necessary elements in their diet that are not easy to find in nature so, when they do find them, they eat a lot of it so as to 'balance' things out. We, humans, no longer need to depend on hunting and foraging for our nutrition so our craving for salt has turned against us and we need to be careful how much we end up consuming. Well, it's the same thing for parrots and protein. Give a parrot a bowl full of seeds and he will choose the ones with the higher protein and eat and eat and eat of them until he is full without looking at the leafy green. But, give, say, a canary -which is a natural seed eater- the same choice and the canary will go for the green first. See what I mean? It's a matter of offering the same amount of that particular dietary requirement that the animal would get in nature. I actually give them more protein during the warm weather months precisely because I want to reproduce as closely as possible the dietary seasons or cycles they follow in nature.
The low protein link to fatty liver disease study - again, you need to look closely at everything in a study. This study did not say that low protein causes fatty liver, it said that fatty liver disease under a low protein diet was caused by a defect in the production of tryglicerides - I quote from the study: "It is concluded that the fatty liver of protein deficiency is predominantly due to a defect in the secretion of hepatic triglycerides" - See the difference?
You are 100% right that it would be very useful to have the gloop recipe analyzed so we could tell exactly all the different values but I'll tell you, the reason why I haven't done it is twofold: lack of funds and the fact that I am constantly changing it so, the results I would get today would not be the same as the ones I will get in two, three, six months time and here is where the lack of funds comes in - because I would not mind having it done once but having it done every few months will not go well with my husband (the one who pays my birds bills -which are pretty high as it is

But, as soon as I have some extra money and have settled on a recipe -at least for a little while

, I will). But the values you give on the gloop recipe don't seem to be right because, for example, kamut is not 2% protein as you have it -neither is wheat- it's actually much higher (between 10 and 14% - see this:
http://www.einkorn.com/wp-content/uploa ... Matrix.pdf). I don't use cereal oats or durum wheat or brown rice, for that matter. And yes, the amino acid score you mention is a good indicator but you need to take into consideration that it's based on the requirements of humans and their ability to digest it. We were not created to live on the same food as parrots so, although I do take it into consideration (and that's why I use all different kinds of grains and seeds and add insect protein to it on the warm weather months), I don't consider it completely relevant for birds.